Learn More
CD8a Monoclonal Antibody (OX8), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
€ 119.00 - € 267.00
Specifications
Antigen | CD8a |
---|---|
Clone | OX8 |
Concentration | 0.2 mg/mL |
Applications | Flow Cytometry |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15350890
|
Affymetrix eBioscience
25-0084-80 |
25 μg |
€ 119.00
25µg |
Estimated Shipment: 13-05-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
15360890
|
Affymetrix eBioscience
25-0084-82 |
100 μg |
€ 267.00
100µg |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
Description: The OX8 monoclonal antibody reacts with rat CD8 alpha. CD8 alpha is a member of the immunoglobulin superfamily which heterodimerizes with CD8 beta to form the CD8 antigen expressed on CD4+CD8+, double-positive thymocytes, the majority of MHC class I-restricted peripheral T cells, and on some macrophages. CD8 binds to MHC class I expressed on the surface of antigen-presenting cells during antigen presentation, and participates in T-cell receptor signal transduction through association with the kinase Lck. Applications Reported: This OX8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX8 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.Specifications
CD8a | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
OX8 | |
Flow Cytometry | |
PE-Cyanine7 | |
Mouse | |
Rat | |
Primary | |
4° C, store in dark, DO NOT FREEZE! |
For Research Use Only.