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CD90.1 (Thy-1.1), APC-eFluor 780, clone: HIS51, eBioscience™

Mouse Monoclonal Antibody

Merk:  Affymetrix eBioscience 47-0900-82

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331640

  • € 259.00 / 100 µg


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Beschrijving

Beschrijving

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor™ emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

CD90.1 (Thy-1.1)
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P01830, P01831
THY1, Thy-1 cell surface antigen, FLJ33325, Thy-1 T-cell antigen, Thy-1 membrane glycoprotein
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine, Rat
Flow Cytometry
HIS51
APC-eFluor 780
Thy1
Liquid
Thy1
IgG2a, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
21838, 24832
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