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  5. Invitrogen™ Human CHI3L1/YKL-40 ELISA Kit

Invitrogen™ Human CHI3L1/YKL-40 ELISA Kit

Product Code. p-7231033
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 15494943

Brand: Invitrogen™ EHCHI3L1

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Description

ELISA

The Human CHI3L1/YKL-40 ELISA quantitates Hu CHI3L1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CHI3L1. Principle of the method The Human CHI3L1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human cartilage glycoprotein 39 (GP-39), also known as YKL-40, is a glycoprotein secreted by articular chondrocytes, synoviocytes and macrophages. Serum and synovial fluid GP-39 levels are elevated in inflammatory diseases and correlate with the degree of joint destruction in rheumatoid arthritis. GP-39 is expressed in articular chondrocytes and synovial cells, as well as in liver, but is undetectable in muscle tissues, lung, pancreas, mononuclear cells and fibroblasts. GP-39 is a candidate autoantigen in rheumatoid arthritis and is important in the capacity of cells to respond to and cope with changes in their environment.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P36222
Assay Range 6.14 to 1500 pg/mL
Assay Sensitivity 6 pg/mL
Calibration Range 6 pg/mL to 1,500 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Kinase & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA pair antibody detects human CHI3L1. Other species not determined.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1116
Gene Symbol ASRT7,CGP-39,GP-39,GP39,HC-gp39,HCGP-3 P,YKL-40,YKL40,YYL-40,hCGP-39
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Angiogenesis
Sample Volume Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3 P, YKL-40, YKL40, YYL-40, hCGP-39
Target CHI3L1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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