Compbead anti-Rat and anti-Hamster Ig κ/Negative Control Compensation Particles Set, BD

The BD CompBeads Compensation Particles Anti-Rat/Hamster Ig, kappa Set are polystyrene microparticles which are used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. The set provides two populations of microparticles, the BD CompBeads Anti-Rat/Hamster Ig, kappa particles, which bind any rat or hamster kappa light chain-bearing immunoglobulin, and the BD CompBeads Negative Control (FBS) Particles, which has no binding capacity. When mixed together with a fluorochrome-conjugated rat or hamster antibody, the BD CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software. Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra). Use of the BD CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy7, APC-Cy7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.

Flow Cytometry