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STEMCELL Technologies Complete Kit for Human Whole Blood CD34+ Cells
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Quantity:
120 mL whole blood
Unit Size:
1 set
Description
- The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat
- First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail (15186C) with antibodies recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b and CD61 surface markers
- CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18066C), which contains an antibody recognizing CD34
- RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation
- The pre-enriched fraction containing CD34+ cells is harvested and then labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet
- Isolated CD34+ cells are immediately available for downstream applications
Specifications
Specifications
| Cell Type | Hematopoietic Stem and Progenitor Cells |
| For Use With (Application) | Cell Isolation |
| Quantity | 120 mL whole blood |
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