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Gibco™ ExpiCHO™ Expression Medium

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells

Brand:  Gibco™ A2910003

4338.00 EUR valid until 2024-06-28
Use promo code "21649" to get your promotional price.



Additional Details : Weight : 10.00000kg

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Product Code. 15461044

  • € 4820.00 / 10 L
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Description

Description

ExpiCHO™ Expression Medium is a GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. It is a core component of the ExpiCHO™ Expression System Kit (Cat. No. A29133) for scalable transient protein production.

ExpiCHO Expression Medium:
  • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
  • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent
  • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL
  • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein
  • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors
  • Does not contain phenol red

    ExpiCHO Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically-defined formulation results in high reproducibility and lot-to-lot reliability. For highest level transient protein expression, the medium is used in combination with a matched culture feed, ExpiCHO™ Feed, which is supplied as part of the ExpiFectamine™ CHO Transfection Kit (Cat. No. A29129). ExpiCHO Expression Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multi-well plates to >10 L in bioreactors.
  • Specifications

    Specifications

    Storage conditions: in the dark at 2-8°C
    Shipping conditions: ambient
    Shelf life: 12 months from date of manufacture
    Animal Origin-free, Chemically-defined, Protein-free, Serum-free
    Liquid
    Serum-free
    No Phenol Red
    ExpiCHO™
    CHO-S, SFM Adapted
    Suspension Cell Culture
    CHO Expression Medium
    GlutaMAX
    Universal Bag
    Room Temperature
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