PeproTech CXCL3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-gamma (CXCL3). Anti-Human GRO-gamma (CXCL3)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human GRO-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-gamma (500-P105) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-gamma. Western Blot: To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P105BT-1MG will be provided as 2 x 500 μg

CXCL3 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils.