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Invitrogen™ Cytochrome C Monoclonal Antibody (6H2), eBioscience™
Mouse Monoclonal Antibody
Brand: Invitrogen™ 14-6601-82
This item is not returnable.
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Description
Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c. Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis, and immunoprecipitation. (Fluorochrome conjugated 6H2 is recommended for use in intracellular flow cytometry.). Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
Specifications
| Cytochrome C | |
| Monoclonal | |
| 0.5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| P62897, P62898, P99999 | |
| Cycs | |
| Affinity chromatography | |
| RUO | |
| 13063, 25309, 54205 | |
| 4°C | |
| Liquid |
| Flow Cytometry, Immunoprecipitation | |
| 6H2 | |
| Unconjugated | |
| Cycs | |
| CG17903; CG17903-PA; CG17903-PB; CRF; CYC; CYC2; Cycs; CYCSA; Cyct-c-p; Cyt c; cyt c-p; cyt.c; CytC; Cyt-c; CYTC2; Cyt-c2; CytC-2; cyt-c-p; cytC-p; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome c proximal; Cytochrome C, expressed in somatic tissues; cytochrome c, somatic; Cytochrome c-2; cytochrome c-like; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; hc1; HCS; l(2)k13905; lethal (2) k13905; LOC100341175; LOC100357840; MGC128356 protein; pDMc01; RH17228p; THC4 | |
| Mouse | |
| 100 μg | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG1 κ |
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