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Thermo Scientific™ DMEM for SILAC

Product Code. 15645729 Shop All Thermo Scientific Products
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Product Code. 15645729 Supplier Thermo Scientific™ Supplier No. 88364
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Description

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ DMEM for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of DMEM for SILAC:

  • Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Related products

  • NeuCode™ Lysine -080, 50 mg (A33613)
  • Fetal Bovine Serum, dialyzed, US origin (26400036)
  • RPMI 1640 Media for SILAC (88365)
TRUSTED_SUSTAINABILITY

Spezifikation

For Use With (Equipment) Mass Spectrometer
Product Type Media
Content And Storage 1 x 500 mL, store at 2–8°C
Label or Dye Mass Isotope of Amino Acid
Shipping Condition Room Temperature
Starting Material Protein Samples
Final Product Type Liquid Medium
Detection Method Mass Spectrometry
Workflow Step Cell Culture
Quantity 500 mL
Form Liquid
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For Research Use Only. Not for use in diagnostic procedures.

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