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  5. Invitrogen™ Mouse DPPIV (DPP4/CD26) ELISA Kit

Invitrogen™ Mouse DPPIV (DPP4/CD26) ELISA Kit

Product Code. 17165719
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 17165719

Brand: Invitrogen™ EMDPP4X5

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Descripción

ELISA

The Mouse DPPIV (DPP4/CD26) ELISA quantitates Ms DPPIV in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms DPPIV. Principle of the method The Mouse DPPIV solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.
TRUSTED_SUSTAINABILITY

Especificaciones

Accession Number P28843
Assay Range 0.41 to 100 ng/mL
Assay Sensitivity 0.45 ng/mL
Calibration Range 0.45 ng/mL to 100 ng/mL
Description DPPIV (DPP4) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Proteases, Peptidases and Inhibitors Protein Family, Cell Cycle and Proliferation Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Dppiv (Dpp4) i
Detectable Analytes DPPIV (DPP4)
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Proteases, Peptidases & Inhibitors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Substrate Type TMB
Sufficient For 5 x 96 assays
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 13482
Gene Symbol Cd26,Dpp-4,THAM
Host Species Mouse
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Cell Cycle & Proliferation
Sample Volume Plasma 5 μL, Serum 5 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP23-87G8.2, Cd26, Dpp-4, THAM
Target DPP4
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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