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  6. Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kits

Product Code. 12760170
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Quantity:
100 Tests
20 Tests
200 Test
200 Tests
300 Tests
50 Tests
Excitation/Emission:
405, 546/450, 647
488, 535/520, 617
650, 535/660, 617
NA, 535/NA, 617
Conjugate:
APC, Propidium Iodide
Biotin, Propidium Iodide
FITC, Propidium Iodide
PCP-eFluor 710
PE, 7-AAD
PE/Cy7
eFluor 450, 7-AAD
Unit Size:
100 tests
20 tests
200 tests
300 tests
50 tests
Each
18 product options available for selection
Product selection table with 18 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity Excitation/Emission Conjugate Unit Size
12760170 50 Tests 488, 535/520, 617 FITC, Propidium Iodide Each
12770170 200 Tests 488, 535/520, 617 FITC, Propidium Iodide Each
15561187 50 Tests 405, 546/450, 647 eFluor 450, 7-AAD 50 tests
15571187 200 Tests 405, 546/450, 647 eFluor 450, 7-AAD 200 tests
12780170 200 Tests 650, 535/660, 617 APC, Propidium Iodide Each
15591187 50 Tests - PCP-eFluor 710 50 tests
15413392 200 Test - PCP-eFluor 710 Each
15581947 100 Tests 488, 535/520, 617 FITC, Propidium Iodide 100 tests
15501957 20 Tests 488, 535/520, 617 FITC, Propidium Iodide 20 tests
15521957 300 Tests 488, 535/520, 617 FITC, Propidium Iodide 300 tests
14819780 50 Tests - PE, 7-AAD 50 tests
15521207 200 Tests - PE, 7-AAD 200 tests
15531207 50 Tests - PE/Cy7 50 tests
15541207 200 Tests - PE/Cy7 200 tests
15551947 100 Tests NA, 535/NA, 617 Biotin, Propidium Iodide 100 tests
15561947 20 Tests NA, 535/NA, 617 Biotin, Propidium Iodide 20 tests
15571947 300 Tests NA, 535/NA, 617 Biotin, Propidium Iodide 300 tests
15581187 50 Tests 650, 535/660, 617 APC, Propidium Iodide 50 tests
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 18 options available.
18 options
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Product Code. 12760170 Supplier Invitrogen™ Supplier No. 88800572
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    € 170.65 / 50 reactions
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Description

Includes

  • Cat. No. 88-8005-72:
  • 10X Binding Buffer
  • Annexin V FITC : 5μL/test, 50 tests Protect from light.
  • Propidium Iodide Staining Solution : 5μL/test, 100 tests Protect from light.
  • Cat. No. 88-8005-74:
  • 10X Binding Buffer
  • Annexin V FITC : 5μL/test, 200 tests Protect from light.
  • Propidium Iodide Staining Solution : 5μL/test, 2x100 tests Protect from light.

Kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane.

Easily distinguish cells undergoing early and late apoptosis with eBioscience and Annexin V Apoptosis Detection kits for flow cytometry analysis. These kits utilize viability dye-conjugated annexin V to label phosphatidylserine (PS), an early marker of apoptosis, on the extracellular membrane. In late-stage apoptosis, as cell integrity is lost, both annexin V and the viability dye traverse and bind PS on the interior of the plasma membrane.

Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled annexin V in a calcium-dependent manner.

In early-stage apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI), 7-AAD, or fixable viability dyes such as eFluor 450. These cells will stain with annexin V but not a viability dye, thus distinguishing cells in early apoptosis. However, in late-stage apoptosis, the cell membrane loses integrity, thereby allowing annexin V to also access PS in the interior of the cell. A viability dye can be used to resolve these late-stage apoptotic and necrotic cells (annexin V, viability dye-positive) from the early-stage apoptotic cells (annexin V positive, viability dye-negative).

Current viability stains available with the eBioscience Annexin V Apoptosis Detection kits include FITC, eFluor 450, allophycocyanin (APC), phycoerythrin (PE), PE-cyanine7, and biotin.

TRUSTED_SUSTAINABILITY

Specifications

Description eBioscience Annexin V Apoptosis Detection Kit FITC
Quantity 50 Tests
Format Kit
Product Type Apoptosis Detection Kit
Content And Storage Contains:
• Annexin V Binding Buffer (10X), 1 bottle (30 mL)
• Annexin V-FITC conjugate, 1 vial (0.25 mL)
• Propidium Iodide Staining Solution, 1 vial (0.5 mL)

Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits. Handling Conditions:
Store at 2-8°C. Avoid temperature fluctuations.
Excitation/Emission 488, 535/520, 617
Product Line eBioscience
Conjugate FITC, Propidium Iodide
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer

Frequently Asked Questions (FAQs)

Can I use eFluor 450-conjugated antibodies with eBioscience Annexin V Apoptosis Detection kits?

Yes, eFluor 450-conjugated antibodies are compatible with eBioscience Annexin V Apoptosis Detection kits.
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