Invitrogen™ EnzChek™ 3C-like Protease (3CLpro) & Papain-like Protease (PLpro) Assay Kits

The EnzChek 3C-like Protease (3CLpro) and Papain-like Protease (PLpro) Assay kits are quick, easy, and direct assays for detecting and quantifying SARS-CoV-2 3-chymotrypsin-like protease (3CLpro) and SARS-CoV-2 papain-like protease (PLPro) activity. These kits can be used to screen and profile inhibitors of 3CLpro and PLpro and include all reagents for the determination of protease activity, including a positive control inhibitor. 3CLpro and PLpro activity can be directly correlated using a 96-well plate and microplate reader.

EnzChek 3CLpro and PLpro Assay kit features include:

• Sensitive — detect as little as 3 ng of 3CLpro or 6 ng of PLpro
• Quick — less than two hours for results
• Simple — easy to use and ideal for high-throughput screening applications

3CLpro, also known as main protease (Mpro), and PLpro are crucial enzymes for processing viral polyproteins, and due to their role in the SARS-CoV-2 virus life cycle have been identified as a promising drug target for blocking viral replication. The homogeneous EnzChek 3CLpro and PLpro Assay kits are ideal for easily screening and quantifying inhibitors of 3CLpro and PLpro.

The EnzChek 3C-like 3CLpro and PLpro Assay kits contain a fluorogenic protease-specific substrate, SARS-CoV-2 3CLpro or PLpro, positive control inhibitor, and assay buffer. Simply add your inhibitor of choice. Protease-catalyzed hydrolysis releases the fluorescently labeled peptide, and protease activity can be directly quantified from the change in fluorescence signal using a microplate reader (ex: 360 nm, em: 460 nm).