EOMES Monoclonal Antibody (Dan11mag), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenpcytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.