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Thermo Scientific™ X-Gal-oplossing, gebruiksklaar

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Merk:  Thermo Scientific™ R0941

Overige gegevens : Gewicht : 1.51950kg

Produktcode. 10508750

  • € 124.00 / 10 ml


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Beschrijving

Beschrijving

Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies. X-Gal Solution, ready-to-use, is a stable, 0.22μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.

Recommended for:

Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.

Note:

Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.

Specificatie

Specificatie

Blauw/witte koloniescreening voor onderscheid tussen recombinante (witte) en niet-recombinante (blauwe) kolonies (1); visualisatie van beta-galactosidase-reporter genexpressie in getransfecteerde eukaryotische cellen; detectie van beta-galactosidase-activiteit in immunologie
X-Gal-oplossing, gebruiksklaar
10 ml
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