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  5. Thermo Scientific™ RsaI (10 U/μL)

Thermo Scientific™ RsaI (10 U/μL)

Product Code. 10334380 Shop All Thermo Scientific Products
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Product Code. 10334380 Supplier Thermo Scientific™ Supplier No. ER1121

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Description

The RsaI restriction enzyme recognizes GT^AC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AfaI, CviQI, RsaNI).

Lambda DNA digested with RsaI, 1.4% agarose, 113 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G T▵A C...3'

3'...C A▵T G...5'

Conditions for 100% Activity

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer

  • RsaI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

  • After 50-fold overdigestion with RsaI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effect

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect
TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Rsa I
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 1,000 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.

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