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Invitrogen™ FGF4 (Cell Lysates) Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHFGF4CLX5
Additional Details : Weight : 0.33820kg
Description
The Human Fibroblast Growth Factor 4 Cell Lysates (Hu FGF4CL) ELISA quantitates Hu FGF4CL in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu FGF4CL. Principle of the method The Human FGF4CL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.Specifications
| P08620 | |
| 50 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Cell Lysates | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 2249 | |
| <12% | |
| Standard, Biotinylated Detection Antibody, Chromogen, Adhesive Plate Covers, SAV-HRP, Wash Buffer, Cell Lysis Buffer, Assay Diluent Concentrate, Stop Solution, Pre-coated 96 well plates | |
| 5 x 96 Tests | |
| Apoptosis | |
| 2°C to 8°C | |
| Human | |
| 4 hr. 45 min. |
| 24.69 pg/mL to 18,000 pg/mL | |
| 50 pg/mL to 18,000 pg/mL | |
| Biotin | |
| Growth Factors & Receptors | |
| The antibody pair provided in this kit recognizes human FGF-4. | |
| Human | |
| Colorimetric Microplate Reader | |
| HBGF-4,HST,HST-1,HSTF1,K-FGF,KFGF | |
| <10% | |
| HRP | |
| RUO | |
| Cell Lysate,20 μ;L | |
| HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF | |
| 1 hr. 20 min. |