FITC Monoclonal Antibody (1F8-1E4), TRITC, Invitrogen™

Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Fluorophore: Tetramethyl rodamine 5 (and 6)-isothiocyanate (TRITC) Amax = 550 nm; Emax = 570 nm Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44 Product #31687 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical, immunoprecipitation and Western blot applications. Restoration and Storage: Store product at 4°C until opened. Restore with 0.35 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C; or as an alternative, aliquot without glycerol and store at -80o or below, avoiding repeated freezing and thawing. Country of Origin: USA

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs μgtraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit,