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Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450

A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.

€ 146.00 - € 414.00

Specifications

Color Blue
Detection Method Fluorescent
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15211938
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Invitrogen™
65-0863-14
100 Tests
€ 146.00
Each
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15331988
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Invitrogen™
65-0863-18
500 Tests
€ 414.00
Pack of 500
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Description

Description

  • Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
  • Thus, using these dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
  • Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
  • After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
  • Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
  • It should be protected from light and moisture
  • Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
Specifications

Specifications

Blue
Viability Assay
Dye
eBioscience™
Fluorescent
Flow Cytometer
405/450
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.