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BD Biosciences™ Fixable Viability Stain 440UV (RUO)

Product Code. 15814959
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Quantity:
200 μg
Unit Size:
Each
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Product Code. 15814959

Brand: BD Biosciences™ 566332

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This item is not returnable. View return policy

Description

Reacts with and covalently binds to cell-surface and intracellular amines.

  • BD Horizon™ Fixable Viability Stain 440UV (FVS440UV) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications.
  • This dye reacts with and covalently binds to cell-surface and intracellular amines.
  • Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells.
  • Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells.
  • The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.
  • BD Horizon™ Fixable Viability Stain 440UV is excited by the UV laser (with an excitation maximum of 338 nm), and has a fluorescence emission maximum of 436 nm.
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Upon arrival, store the dry dye desiccated and protected from light at -80°C until use. After reconstitution with DMSO, store the Stock Solution at -20°C in small aliquots. Do not use reconstituted dye after 90 days of storage. Please discard the dye solution after 90 days post reconstitution with DMSO.
Format Dry, dessicated
Quantity 200 μg
Product Type Fixable Viability Stain

Safety and Handling

compliance-icons
Product Identifier
  • BD Horizon Fixable Viability Stain 440UV
 Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 1
 Hazard Statement
  • H318-Causes serious eye damage.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contains : NHS ester
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