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Thermo Scientific™ GeneJET Genomic DNA Purification Kit
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Description
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes
Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting
Specifications
Specifications
| Column Type | Spin Column |
| Content And Storage | • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening • 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening • 55 mL Digestion Solution; room temperature • 2 x 60 mL Lysis Solution; room temperature • 40 mL Wash Buffer I (concentrated); room temperature • 40 mL Wash Buffer II (concentrated); room temperature • 150 mL Elution Buffer; room temperature • 250 GeneJET Purification Columns with Collection Tubes; room temperature • 250 Collection Tubes; room temperature |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples |
| Elution Volume | 200 μL |
| Final Product Type | gDNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 250 Preps |
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Safety and Handling
- GeneJET Genomic DNA Purification Kit
- Danger
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Proteinase, tritirachium album serine, glycerine, Sodium lauryl sulfate, Guanidine hydrochloride
For Research Use Only. Not for use in diagnostic procedures.
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