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Gibco™ HEPES (1 M)
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Product Code. 12509079
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Product Code. 12509079 Supplier Gibco™ Supplier No. 15630080
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Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, relatively stable, and has limited interference in key processes.

Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator.

Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a good buffering choice for many cell culture systems because it:

  • Is membrane impermeable
  • Has limited effect on biochemical reactions
  • Is chemically and enzymatically stable
  • Has a very low visible and UV light absorbance

cGMP manufacturing and quality system

For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY, and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

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Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Zwitterionic Organic Chemical Buffer
CAS 7365-45-9
Color Clear
Concentration 1 M
For Use With (Application) Chromatin Biology
pH 7 to 7.5
Physical Form Liquid
Quantity 100 mL
Solution Type Buffer
Formulation N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid
Sterility Sterile
Sterilization Method Sterile-filtered
Molecular Weight (g/mol) 238.3 g/mol
Grade Biochemical
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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