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Invitrogen™ Goat anti-Rabbit IgG (H+L) Secondary Antibody, Biotin
Description
Product # 31820 has been successfully used in Western blot, IF, ICC, ISH, and IHC applications. Product # 31820 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. This antibody cross-reacts with rabbit IgM (25%). However, this antibody may cross-react with immunoglobulins from other species.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Specifications
Specifications
| Antigen | Rabbit IgG (H+L) |
| Applications | ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot, Immunocytochemistry |
| Classification | Polyclonal |
| Concentration | 1.5 mg/mL |
| Conjugate | Biotin |
| Formulation | PBS with 3mg/mL BSA and 0.08% sodium azide; pH 7.8 |
| Host Species | Goat |
| Purification Method | Affinity chromatography |
| Quantity | 1.5 mg |
| Regulatory Status | RUO |
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