missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Assays
  5. Bioluminescence Assays and Reagents
  6. 12172230

Thermo Scientific™ pCMV-Green Renilla Luc Vector for Luciferase Assays

Product Code. 12172230 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
10 μg
Conditionnement:
10µg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Code produit Quantity unitSize
12172230 10 μg 10µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.
Code produit 12172230 Fournisseur Thermo Scientific™ Code fournisseur 16153

Please to purchase this item. Need a web account? Register with us today!

Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

Description

Drive Green Renilla luciferase expression as a reporter for gene regulation studies using vectors with a multiple cloning site (MCS) or control promoter (CMV or TK).

The Thermo Scientific™ pMCS-Renilla Luc Vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.

The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization
TRUSTED_SUSTAINABILITY

Spécification

Description pCMV-Green Renilla Luc Vector
Type Luc Vector
Cloning Method Restriction Enzyme/MCS
Delivery Type Transfection
Quantity 10 μg
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Reporter Gene Green Renilla Luciferase
Selection Agent (Eukaryotic) Puromycin
Product Line Pierce
For Use With (Application) Reporter Assays
Promoter CMV
Afficher plus Afficher moins

For Research Use Only. Not for use in diagnostic procedures.

Nom du produit
Sélectionnez un problème

En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.