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Thermo Scientific Chemicals Guanidine hydrochloride, Molecular Biology Grade
Description
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Specifications
| Melting Point | 178°C to 185°C |
| Color | White |
| Density | 1.34 |
| Quantity | 500 g |
| Beilstein | 3591990 |
| Sensitivity | Hygroscopic |
| Merck Index | 144562 |
| Solubility Information | Freely soluble in water |
| Formula Weight | 95.53 |
| Grade | Molecular Biology |
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Safety and Handling
- Guanidine hydrochloride, Molecular Biology Grade
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- MIXTURE LIST-Contains : guanidine hydrochloride
RUO – Research Use Only