Invitrogen™ HCoV-NL63 Spike S1/S2 Recombinant Rabbit Monoclonal Antibody (279)

Description

Recognize the Spike of HCoV-NL63 in ELISA. Doesn't recognize the Spike of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-229E, HCoV-HKU1 (isolate N5), HCoV-OC43.. Has cross-reactivity in ELISA with: HCoV-NL63 Spike S1+S2 ECD-His; No cross-reactivity in ELISA with: SARS-CoV-2 Spike S1-His; SARS-CoV-2 Spike S1 NTD-Fc & AVI; SARS-CoV-2 Spike S1 NTD-His & AVI; SARS-CoV-2 Spike RBD-His; SARS-CoV-2 Spike S1+S2 ECD-His; SARS-CoV-2 Spike S2 ECD-His; SARS-CoV Spike S1+S2 ECD-His; MERS-CoV Spike S1+S2 ECD-His; HCoV-229E Spike S1+S2 ECD-His; HCoV-OC43 Spike S1+S2 ECD-His; HCoV-HKU1 (isolate N5) Spike S1+S2 ECD-His.

HCoV-NL63 Spike S1/S2 refers to the specific regions of the spike (S) protein of the Human Coronavirus NL63 (HCoV-NL63), which is a virus known to cause respiratory infections, including the common cold, particularly in children, the elderly, and immunocompromised individuals. The spike protein is essential for viral entry into host cells and is composed of two subunits: S1, which contains the receptor-binding domain (RBD) responsible for attaching to the host cell receptor angiotensin-converting enzyme 2 (ACE2), and S2, which facilitates the fusion of the viral and host cell membranes. The S1/S2 cleavage site is a critical juncture where host proteases cleave the spike protein, activating it for membrane fusion and subsequent viral entry. Understanding the structure and function of these regions is crucial for developing targeted therapies and vaccines.