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Cytiva His Mag Sepharose Excel (10%)
Designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.
Brand: Cytiva 29358345
Additional Details : CAS : 64-17-5 Weight : 0.11500kg
Description
His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
- Robust parallel screening with high reproducibility
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Capture histidine-tagged protein from different sample volumes (low microliter to high milliliter scale)
- Visible and dense Sepharose based magnetic beads give increased simplicity for handling
- High density of beads ensures rapid capture by magnetic devices
Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.
The base of the matrix is based on Sepharose resin, incorporated with magnetite.
Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.
Specifications
| < At least 10 mg (histidine)6-tagged protein/ml sedimented medium | |
| 4°C to 30°C, 20% ethanol, 10% medium slurry | |
| Immunoprecipitation and purification of biotinulated biomolecules |
| Highly cross-linked spherical agarose including magnetite | |
| 100 mL | |
| IMAC immobilized with Ni |