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Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 0.2 mL

Product Code. 11802114 Shop All Thermo Scientific Products
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25 Column Kit
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Pack of 25
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11802114 25 Column Kit Pack of 25
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Product Code. 11802114 Supplier Thermo Scientific™ Supplier No. 88227

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Description

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

  • Versatile – purify proteins using native or denaturing conditions

  • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

  • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

  • Cost-effective – reuse the same batch of resin at least five times

  • Easy to use – pre-formulated buffers available for kit formats

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 0.2 mL/Column
Description HisPur Ni-NTA Spin Purification Kit, 0.2mL
Content And Storage Sufficient For: Purifying up to 12 mg of His-tagged protein per column
• HisPur Ni-NTA Spin Columns, 0.2 mL, 25 columns
• Phosphate-buffered Saline (10X), 30 mL
• Imidazole (2M), 8 mL
• Collection Tubes, 80 tubes
• Column Plugs, 25 plugs

Store at 4°C.
Product Line HisPur
Quantity 25 Column Kit
Target His-Tagged Protein
Type Spin Column Kit
Stationary Phase Divalent Nickel, Metal Chelate
Sufficient For 12 mg of His-Tagged Protein per Column
Format Kit, Microcentrifuge, Spin Column
Column Type Affinity, Agarose Resin
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Safety and Handling

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Product Identifier
  • X25 HISPUR NI-NTA PURIFICATION KIT
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Carcinogenicity Category 1A
  • Serious eye damage/eye irritation Category 2
  • Reproductive toxicity Category 1B
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 B
  • Skin sensitiser Category 1
  • Specific target organ toxicity after repeated exposure Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350-May cause cancer.
  • H360D-May damage the unborn child.
  • H360FD-May damage fertility. May damage the unborn child.
  • H373-May cause damage to organs.
  • H412-Harmful to aquatic life with long lasting effects.
 Precautionary Statement
  • P101-If medical advice is needed, have product container or label at hand.
  • P102-Keep out of reach of children.
  • P103-Read label before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulfuric acid, nickel(2+) salt, hydrate,sodium chloride, disodium hydrogenorthophosphate, imidazole

For Research Use Only. Not for use in diagnostic procedures.

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