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Cytiva HisTrap HP, 100 Columns
Ensure reliable, high-yield capture of histidine-tagged proteins in IMAC. GE Healthcare HisTrap HP, 100 Columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification by immobilized metal ion affinity chromatography (IMAC).
€ 2882.00 - € 10201.00
Specifications
Binding Capacity | At least 40 mg histidine-tagged protein/mL medium, Protein binding capacity is protein-to-protein dependent. |
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Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
Material | Polypropylene (PP) |
Matrix | Highly cross-linked agarose, 6% |
Product Code | Brand | Quantity | Bed Volume | Price | Quantity & Availability | ||||
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Product Code | Brand | Quantity | Bed Volume | Price | Quantity & Availability | ||||
11555674
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Cytiva
17-5247-05 |
100 x 1 mL | 1 mL |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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11880073
|
Cytiva
17-5248-05 |
100 x 5 mL | 5 mL |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
HisTrap HP 1ml and 5ml columns are designed for one-step purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose High Performance to ensure reliable capture of target protein in repeated IMAC purifications. The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield. At the same time, it greatly expands the range of suitable operating conditions.- High binding capacity—at least 40mg/ml chromatography medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump or chromatography system, such as ÄKTA design
- Can be used for purification of a range of tagged proteins
- 100-column bulk pack for convenience and economy
Specifications
At least 40 mg histidine-tagged protein/mL medium, Protein binding capacity is protein-to-protein dependent. | |
4 mL/min. (1 mL), 5 mL/min. (5 mL) | |
Highly cross-linked agarose, 6% | |
5 bar (0.5 MPa, 70 psi) | |
Pre-packed Column |
HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. | |
Polypropylene (PP) | |
4°C to 30°C, 20% Ethanol | |
34 μm |