R&D Systems™ Human Active MMP-1 Fluorokine E Kit

The Fluorokine E Human Active MMP-1 kit combines the specificity of a monoclonal antibody that captures both pro and active MMP-1 forms and the sensitivity of fluorescence. The kit is designed to measure the levels of both endogenous active MMP-1 in cell culture supernates, serum, and plasma and the MMP-1 in these samples that can be activated by APMA in the assay procedure. The measured MMP-1 activity may reflect the balance of MMP-1 and its inhibitor TIMPs. Therefore, it is recommended that the levels of TIMPs be determined in order to interpret the results properly. For example, TIMP-1 inhibits the activity of MMP-1 through binding of the N-terminal domain of the TIMP-1 to the active site of MMP-1 (8). The Quantikine Human TIMP-1 Immunoassay (R&D Systems Catalog # DTM100) can be used to measure TIMP-1 concentrations in these samples.