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Invitrogen™ Human B3GNT1 (aa 40-183) Control Fragment Recombinant Protein

Product Code. 30207395
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Quantity:
100 μL
Unit Size:
100µL
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30207395 100 μL 100µL
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Product Code. 30207395 Supplier Invitrogen™ Supplier No. RP89938

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Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (98%), Rat (98%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53307 (PA5-53307. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Beta-1,4-glucuronyltransferase involved in O- mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O- mannosyl glycan (PubMed:25279699, PubMed:25279697). Phosphorylated O-mannosyl glycan is a carbohydrate is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699, PubMed:25279697). Required for axon guidance; via its function in O-mannosylation of alpha- dystroglycan (DAG1).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O43505
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 11041
Name Human B3GNT1 (aa 40-183) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 1500032M01Rik; B3GNT1; B3GN-T1; B3GNT1 (HGNC:15685); B3gnt6; B4GAT1; beta-1,3-N-acetylglucosaminyltransferase bGnT-6; beta-1,4-glucuronyltransferase 1; BETA3GNT1; BETA3GNTI; EC 2.4.1.149; I-beta-1,3-N-acetylglucosaminyltransferase; iGAT; iGNT; MDDGA13; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
Common Name B3GNT1
Gene Symbol B4GAT1
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence EEQDQYFEFFPPSPRSVDQVKAQLRTALASGGVLDASGDYRVYRGLLKTTMDPNDVILATHASVDNLLHLSGLLERWEGPLSVSVFAATKEEAQLATVLAYALSSHCPDMRARVAMHLVCPSRYEAAVPDPREPGEFALLRSCQ
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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