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Invitrogen™ Human eIF3f (aa 104-174) Control Fragment Recombinant Protein

Product Code. 30206841
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Quantity:
100 μL
Unit Size:
100µL
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30206841 100 μL 100µL
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Product Code. 30206841 Supplier Invitrogen™ Supplier No. RP100070

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Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (100%), Rat (100%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-61918 (PA5-61918. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O00303
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 8665
Name Human eIF3f (aa 104-174) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 0610037M02Rik; AA409853; deubiquitinating enzyme eIF3f; eIF3 p47; eIF-3-epsilon; eIF3-epsilon; EIF3F; eIF3-p47; Eif3s5; eukaryotic translation initiation factor 3 subunit 5; eukaryotic translation initiation factor 3 subunit 5 epsilon; eukaryotic translation initiation factor 3 subunit F; eukaryotic translation initiation factor 3, subunit 5 (epsilon); eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47 kD); eukaryotic translation initiation factor 3, subunit 5 epsilon, 47 kDa; eukaryotic translation initiation factor 3, subunit F
Common Name eIF3f
Gene Symbol EIF3F
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence DSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYA
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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