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  5. Invitrogen™ Human hCG alpha ELISA Kit

Invitrogen™ Human hCG alpha ELISA Kit

Product Code. 15484943
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 15484943

Brand: Invitrogen™ EHCG

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Description

ELISA

The Human Chorionic Gonadotropin (hCG) ELISA quantitates Hu CG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CG. Principle of the method The Human CG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Luteinizing hormone (LH, also known as lutropin) is a tropic hormone which modulates the secretory activity of other endocrine glands. It is produced in the anterior hypophysis of the pituitary gland. The glycoprotein hormone, LH, like follicle stimulating hormone and thyroid stimulating hormone, is composed of a common alpha-subunit but also a specific beta-subunit, which characterizes each of these hormones.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01215
Assay Range 54.87 to 40,000 pg/mL
Assay Sensitivity 50 pg/mL
Calibration Range 50 pg/mL to 40000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 1081
Gene Symbol CG-ALPHA,FSHA,GPHA1,GPHa,HCG,LHA,TSHA
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Apoptosis
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP11-156F23.1, CG-ALPHA, FSHA, GPHA1, GPHa, HCG, LHA, TSHA
Target hCG alpha
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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