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Proteintech™ Human IL-31 ELISA Kit

Código de producto. 16871496 Tienda Proteintech Productos
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Target Species:
Human
Tamaño de la unidad:
96 tests
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Código de producto. Target Species Tamaño de la unidad
16871496 Human 96 tests
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Código de producto. 16871496 Proveedor Proteintech™ N.º de proveedor KE0008696T
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Estimated Shipment: 16-11-2026
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Descripción

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human IL31

  • Recognizes human IL-31
  • IL-31 (Interleukin-31) is a four-helix bundle cytokine, expressed by several types of cells under normal and disease state (activated CD4+T cells, mast celles, monocytes, macrophages, immature and mature monocyte-derived dendritic cells)
    • Signals through heterodimeric receptor complex consisting of IL-31 receptor alpha and oncostatin M receptor
    • Plays predominant function in mediation of inflammatory and lymphoma-associated itch
    • Increased IL-31 expression has detached in inflamed tissues from patients with atopic dermatitis, bowel diseases, allergic asthma and rhinitis

Process:

  • Antibody specific for human IL-31 pre-coated onto microwells
  • Human IL-31 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human IL-31 is added to detect captured human IL-31 protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Especificaciones

Accession Number NP_001014358
Assay Range 31.25 to 2000 pg/mL
Assay Sensitivity 7.4 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 386653
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID Q6EBC2
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Seguridad y manipulación

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Nombre del producto
  • 96TEST Human IL-31 ELISA Kit
 Palabra de advertencia
  • Warning
 Clasificación
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Indicaciones de peligro
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Consejos de prudencia
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Otros peligros
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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