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Invitrogen™ Human IL-9 ELISA Ready-SET-Go!™ Kit

Specifically engineered for accurate and precise measurement of human IL-9 protein levels from samples including serum, plasma, and supernatants from cell cultures.

Merk:  Invitrogen™ 88-7958-86

Overige gegevens : Gewicht : 0.47500kg

 Bekijk meerdere types van dit product via de

Produktcode. 15541187

  • € 437.00 / 10 stuks


Geschatte verzenddatum
bij leverancier 01-04-2019
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Waarschuwingen:

Contains Azide


Inclusief: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Beschrijving

Beschrijving

IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.
Specificatie

Specificatie

P15248
Biotin
3578
Colorimetric Microplate Reader
ELISA
RUO
Plasma, Serum, Supernatants
Human
1 hr 45 min
Human
Interleukin-9
1-100 pg/mL
ELISA
96-well plates
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 µM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 plates (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
10 x 96 tests
1 pg/mL
Plasma (100μL), Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
IL9
HRP
Video's

For Research Use Only