missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ p53 Human ELISA Kit

For the quantitative detection of human p53.

€ 634.00 - € 4325.00

Specifications

Accession Number P04637
Assay Range 0.78-50 U/mL
Assay Sensitivity 0.33 U/mL
Conjugate Biotin
Product Type ELISA Kit
View More Specs

Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human p53
Biotin-Conjugate anti-human p53 monoclonal antibody
Streptavidin-HRP
Human p53 Standard lyophilized, 100 U/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15541837
View Documents Promotion Details
Invitrogen™
BMS256
96 Tests
€ 634.00
96 tests
View alternative products
Save Up to 
»
Estimated Shipment: 10-04-2024
to see stock.
Add to basket
 
15551837
View Documents Promotion Details
Invitrogen™
BMS256TEN
10 x 96 Tests
€ 4325.00
Pack of 10
View alternative products
Save Up to 
»
Estimated Shipment: 11-04-2024
to see stock.
Add to basket
 
Description

Description

The Human p53 ELISA quantitates Hu p53 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu p53. Principle of the method The Human p53 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P04637
0.33 U/mL
ELISA Kit
Human
Colorimetric Microplate Reader
BCC7,LFS1,P53,TRP53
5.5%
HRP
Serum, 50 μL; Supernatant, 50 μL
Tumor suppressor p53, TP53, Li-Fraumeni syndrome
1 hr. 20 min.
0.78-50 U/mL
Biotin
Serum, Supernatant
ELISA
7157
8.9%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Human
3 hr. 10 min.
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only