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Proteintech™ Human SERPINA1 ELISA Kit

Product Code. 16851586 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
Product Code. Target Species unitSize
16851586 Human 96 tests
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Product Code. 16851586

Brand: Proteintech™ KE0017896T

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This item is not returnable. View return policy

Description

KE00178 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA1

  • The assay recognizes human SERPINA1. An antibody specific for SERPINA1 has been pre-coated onto the microwells
  • The SERPINA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SERPINA1 is added to detect the captured SERPINA1 protein.
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.
  • Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
  • SERPINA1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors. It is an acute phase glycoprotein mainly (by 80%) synthesized in human liver and is a major blood protein after albumin and the Immunoglobulins.
  • SERPINA1 plays a key antinflammatory role to protect tissues against proteolytic digestion by elastase secreted by neutrophils. In patients with carcinomas, it was reported that SERPINA1 promotes invasive and metastatic capacity in lung cancer, gastric cancer, and colorectal carcinomas.
  • Several studies demonstrated that higher levels of SERPINA1 correlate with more advanced cancer stages. Defects in this gene can cause emphysema or liver disease.

Specifications

Accession Number BC011991
Assay Range 1.56 to 100 ng/mL , 6.25 to 400 ng/mL
Assay Sensitivity 0.2 ng/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 5265
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P01009
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Safety and Handling

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Product Identifier
  • 96TEST Human SERPINA1 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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