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R&DSystems™ Recombinant Human Dkk-1 Protein, CF

Product Code. 30040194
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Quantity:
1 mg
100 μg
20 μg
250 μg
50 μg
500 μg
Unit Size:
100µg
1mg
20µg
250µg
500µg
50µg
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Product Code. 30040194

Brand: R&DSystems™ 11495DK050

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Description

Recombinant Protein

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 10.0-250 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O94907.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 22943
Molecular Weight (g/mol) M.W.-observed: 33-40 kDa, under reducing conditions., M.W.-theroretical: 26 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Dkk-1 protein Thr32-His266
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like
Conjugate Unconjugated
Reconstitution Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Dkk-1
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