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Gibco™ Human Wnt-1 Recombinant Protein

Product Code. 10782144
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10 μg
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Each
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10782144 10 μg Each
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Product Code. 10782144 Supplier Gibco™ Supplier No. PHC1804

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Description

Recombinant Protein

Carrier-Free Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 6 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P04628
For Use With (Application) Bioactivity
Formulation Protein with no preservative
Gene ID (Entrez) 7471
Molecular Weight (g/mol) 34.8 kDa
Name Human Wnt-1
Purification Method Purified
Quantity 10 μg
Storage Requirements -20°C
Regulatory Status RUO
Endotoxin Concentration <0.1 ng/μg
Gene Alias BMND16; INT1; Int-1; murine mammary tumor virus integration site; OI15; Proto-oncogene Int-1; proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; proto-oncogene Wnt-1; sw; swaying; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1); Wnt family member 1; Wnt1; Wnt-1
Common Name WNT1
Gene Symbol WNT1
Biological Activity ED50 = 1.5 - 2.5 ng/mL; determined by the dose-dependent enhancement of alkaline phosphatase production by murine ATDC5 cells treated with BMP-2 (200 ng/mL).
Product Type Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Sequence Human Wnt-1 recombinant protein contains 343 amino acids
Content And Storage -20°C
Activity ED50 = 1.5 - 2.5 ng/mL; determined by the dose-dependent enhancement of alkaline phosphatase production by murine ATDC5 cells treated with BMP-2 (200 ng/mL).
Shipping Condition Wet Ice
Expression System E. coli
Protein Form Recombinant
Form Lyophilized
Protein Subtype Wnt Proteins & Receptors
Research Category Signal Transduction, Stem Cell Research, Differentiation, Oncology
Product Line Gibco
Purity or Quality Grade ≥98% by SDS-PAGE and HPLC
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