Invitrogen™ IDH1 Recombinant Rabbit Monoclonal Antibody (5S2U7)

Description

Positive test controls include: 293T, HepG2, A-549, MCF7, Mouse testis, Mouse brain, Mouse kidney, Rat liver. The target is usually found in the following locations: Cytoplasm, Peroxisome. Immunogen sequence: RNILGGTVFR EAIICKNIPR LVSGWVKPII IGRHAYGDQY RATDFVVPGP GKVEITYTPS DGTQKVTYLV HNFEEGGGVA MGMYNQDKSI EDFAHSSFQM A.

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.