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  5. Invitrogen™ Human IGFBP1 ELISA Kit

Invitrogen™ Human IGFBP1 ELISA Kit

Product Code. 15495013
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 15495013

Brand: Invitrogen™ EHIGFBP1

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This item is not returnable. View return policy

Description

ELISA

The Human Insulin-like Growth Factor Binding Protein 1 (Hu IGFBP1) ELISA quantitates Hu IGFBP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGFBP1. Principle of the method The Human IGFBP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P08833
Assay Range 2.74 to 2000 pg/mL
Assay Sensitivity 5 pg/mL
Calibration Range 5 pg/mL to 2000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3484
Gene Symbol AFBP,IBP1,IGF-BP25,PP12,hIGFBP-1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Signal Transduction
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym tcag7.702, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1
Target IGFBP-1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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