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  5. Invitrogen™ Human IgG1 ELISA Kit

Invitrogen™ Human IgG1 ELISA Kit

Product Code. 17185469
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
This item is not returnable. View return policy

Product Code. 17185469

Brand: Invitrogen™ EHIGG1X5

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Description

ELISA

The Human Immunoglobulin G1 (Hu IgG1) ELISA quantitates Hu IgG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IgG1. Principle of the method The Human IgG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human IgG1, also known as human immunoglobulin G1, is a subclass of human IgG antibodies that is produced by the immune system in response to antigens. It is the most abundant subclass of IgG in the blood and plays a key role in defense against pathogens. IgG1 works by binding to specific antigens and promoting their clearance from the body through various mechanisms, including phagocytosis and complement activation. IgG1 is also involved in other immune responses, such as antibody-dependent cellular cytotoxicity and neutralization of toxins. Human IgG1 can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious and autoimmune diseases.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.05 to 40 ng/mL
Assay Sensitivity 50 pg/mL
Calibration Range 0.05 ng/mL to 40 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity Detect human IgG1. No cross reactivity was found againt IgG2, IgG3, IgG4, IgM, IgA, IgD and IgE
Sufficient For 5 x 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Research Discipline Biomarker-Related Pathways
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target Human IgG1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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