missing translation for 'onlineSavingsMsg'
Learn More

Rat IgG1 kappa, APC, clone: eBRG1, Isotype Control, eBioscience™

Rat Isotype Control

Brand:  Affymetrix eBioscience 17-4301-83

Additional Details : Weight : 1.00250kg

 View more versions of this product

Product Code. 15226324

  • € 316.00 / Each


Estimated Shipment
27-05-2021
Add to basket

Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, This can be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Rat IgG1 kappa
Control
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
IgG1, IgG1k
IgG1, kappa, kappa
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Control, Flow Cytometry
eBRG1
APC
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Not Applicable
Videos
SDS
Documents

For Research Use Only.