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IgM Rat anti-Mouse, Clone: II/41, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5790-85

Additional Details : Weight : 0.01000kg

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Product Code. 15227477

  • € 148.00 / 500µg


Estimated Shipment
23-06-2021
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Description

Description

Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

IgM
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
ELISA, Flow Cytometry, Immunohistochemistry (Frozen)
II/41
Unconjugated
Ighm
Ighm
IgG2a, kappa
500 μg
4° C
Primary
16019
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SDS
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For Research Use Only.