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IgM Monoclonal Antibody (II/41), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

€ 211.00 - € 347.00

Specifications

Antigen IgM
Clone II/41
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15350530
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Affymetrix eBioscience
15-5790-81
50 μg
€ 211.00
50µg
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Estimated Shipment: 09-04-2024
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15360530
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Affymetrix eBioscience
15-5790-82
100 μg
€ 347.00
100µg
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Description

Description

Description: The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis. Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
Specifications

Specifications

IgM
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH
Ighm
Primary
4° C, store in dark, DO NOT FREEZE!
Ighm
II/41
Flow Cytometry
PE-Cyanine5
Rat
Mouse
P01872
16019
IgG2a κ
Affinity chromatography
Antibody
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Product Certifications

For Research Use Only.