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  5. Invitrogen™ Canine IL-10 ELISA Kit

Invitrogen™ Canine IL-10 ELISA Kit

Product Code. 15484873
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Quantity:
5 x 96 Tests
96 Tests
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1 set
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15484873 96 Tests 1 set
17124819 5 x 96 Tests Each
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Product Code. 15484873 Supplier Invitrogen™ Supplier No. ECIL10

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Description

ELISA

The Canine Interleukin 10 (Dog IL-10) ELISA quantitates Dog IL-10 in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog IL-10. Principle of the method The Canine IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P48411
Assay Range 0.11 to 80 ng/mL
Assay Sensitivity 0.1 ng/ml
Calibration Range 0.1 ng/ml to 80 ng/ml
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detect canine IL-10. Other species not determined yet.
Sufficient For 96 assays
Target Species Canine
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 403628
Gene Symbol IL-10
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Immunology
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym IL-10
Target IL-10
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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