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IL-2, PE-Cyanine7, clone: JES6-5H4, eBioscience™

Rat Monoclonal Antibody

Merk:  Affymetrix eBioscience 25-7021-80

Overige gegevens : Gewicht : 0.25000kg

 Bekijk meerdere types van dit product via de

Produktcode. 15331000

  • € 87.50 / 25 µg


Geschatte verzenddatum
bij leverancier 19-12-2018
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Beschrijving

Beschrijving

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17kDa T cell growth factor and a major immunoregulatory cytokine.Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specificatie

Specificatie

IL-2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P04351
Interleukin-2, T-cell growth factor, TCGF, Aldesleukin
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
JES6-5H4
PE-Cyanine7
Il2
Liquid
Il2
IgG2b, kappa, kappa
25μg
4° C, store in dark, DO NOT FREEZE!, handling and experimental procedures.
Primary
16183
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For Research Use Only.