IL-2, PE-Cyanine7, clone: JES6-5H4, eBioscience™
Rat Monoclonal Antibody
Merk: Affymetrix eBioscience 25-7021-80
Overige gegevens : Gewicht : 0.25000kg
BeschrijvingDescription: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17kDa T cell growth factor and a major immunoregulatory cytokine.Applications Reported: This JES6-5H4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This JES6-5H4 antibody has been tested by intracellular staining and flow cytrometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Interleukin-2, T-cell growth factor, TCGF, Aldesleukin|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.