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IL-23 p19 Monoclonal Antibody (G23-8), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7232-81
Additional Details : Weight : 0.01000kg
Description
Description: The G23-8 antibody reacts with the p19 subunit of mouse IL-23. The G23-8 antibody was generated from immunization with authentic, insect cell-expressed, recombit mouse IL-23 heterodimer. The G23-8 antibody can specifically neutralize IL-23 bioactivity with no effect on IL-12 p70 bioactivity. The use of a p19-specific capture antibody and a p40-specific detection antibody yields an IL-23 ELISA which is exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/mL with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/mL with no cross reactivity observed; all values were at the limit of detection of the assay. For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! is available (88-7120). IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g.
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.Specifications
IL-23 p19 | |
Monoclonal | |
1 mg/mL | |
PBS with no preservative; pH 7.2 | |
Q9EQ14 | |
IL23A | |
Affinity chromatography | |
RUO | |
83430 | |
4° C | |
Liquid |
ELISA, Functional Assay, Neutralization, Western Blot | |
G23-8 | |
Functional Grade | |
IL23A | |
il 23; IL 23 A; IL12B; Il-12b; Il12p40; Il-12p40; il23; IL-23; IL-23 subunit alpha; IL23A; IL-23A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 12B; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; P19; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798 | |
Rat | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.