IL-23 p19 Rat anti-Mouse, Functional Grade, Clone: G23-8, eBioscience™
Rat Monoclonal Antibody
Merk: Affymetrix eBioscience 16-7232-81
Overige gegevens : Gewicht : 0.01000kg
BeschrijvingMouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naive and memory T cells, as compared to IL-12. Applications Reported: The G23-8 antibody has been reported for use as the capture antibody in mouse IL-23 ELISA, for Western blotting, and for neutralization of mouse IL-23 bioactivity. Applications Tested: The Functional Grade purified format of the G23-8 antibody has been tested as the capture antibody in an IL-23 ELISA assay (in conjunction with the biotinylated C17.8 antibody) and for neutralization of IL-23 biological activity. For in vitro neutralization, the Functional Grade purified G23-8 antibody at 1.25 μg/mL has been found to neutralize by 50% the biological effect of 0.1 ng/mL mouse IL-23 in an assay of IL-17 secretion by mouse splenocytes. The G23-8 antibody has no effect on the bioactivity of mouse IL-12 p70. For neutralization of both IL-12 p70 and IL-23 p19p40, the anti-IL-12/IL-23 p40 antibody, clone C17.8, is recommended. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. Astandard curve consisting of doubling dilutions of the Recombinant standard over the range of 4000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB, rather than ABTS, should be used as a substrate to achieve this sensitivity level. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. The p19 shares sequence similarity with IL-6 subfamily members and is distantly related to the p35 subunit of IL-12. It shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a biologically active, composite cytokine, IL-23. IL-23 shares some in vivo functions with IL-12, including the activation of the transcription factor Stat4. Similar to IL-12, human IL-23 stimulates IFN-g production and proliferation of PHA blast T cells, as well as in CD45 RO (memory) T cells. Ubiquitous transgenic expression of the IL-23 subunit p19 induces multiorgan inflammation, runting, infertility and premature death.
|PBS with no preservative; pH 7.2|
|IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF|
|ELISA, Functional Assay, Neutralization, Western Blot|
For Research Use Only.