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PeproTech IL-3 Polyclonal Antibody, PeproTech®, Invitrogen™

Product Code. 17805833
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17865813 50 μg 50µg
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Product Code. 17805833 Supplier PeproTech Supplier No. 500P531MG

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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-3 matrix. Sandwich ELISA: To detect Murine IL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-3 (500-P53Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-3. Western Blot: To detect Murine IL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IL-3
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene IL3
Gene Accession No. P01586
Gene Alias BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF
Gene Symbols IL3
Host Species Rabbit
Immunogen E.coli-derived Recombinant Murine IL-3.
Purification Method Antigen affinity chromatography
Quantity 1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 16187
Target Species Mouse
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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