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  5. Cytiva Single Cell GenomiPhi™ DNA Amplification Kit

Cytiva Single Cell GenomiPhi™ DNA Amplification Kit

Product Code. 15301652 Shop All Cytiva Products
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No. of Reactions:
25
100
Unit Size:
100 reactions
25 reactions
This item is not returnable. View return policy

Product Code. 15301652

Brand: Cytiva 29108107

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This item is not returnable. View return policy

Description

Includes

Single Cell GenomiPhi Reaction buffer 1 x 275µL, Single Cell GenomiPhi Enzyme mix 1 x 25µL, Single Cell GenomiPhi Amplification mix 1 x 25µL, Single Cell GenomiPhi DTT Solution 1 x 100µL, Single Cell GenomiPhi Lysis buffer 1 x 100µL and Single Cell GenomiPhi Neutralisation buffer 1 x 100µL

Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

  • For whole genome amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
  • Reaction time: 2 to 4 hours
  • Product average size: >10kb
Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
Description Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
Final Product Type illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
For Use With (Application) Genomic DNA Amplification
Includes Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
No. of Reactions 25
Product Type illustra Single Cell GenomiPhi DNA Amplification Kit
Size >10kb
Test Time 2 to 4 hours

Safety and Handling

compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Skin corrosion/irritation Category 1
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H314-Causes severe skin burns and eye damage.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : potassium hydroxide
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